Antimicrobial Effect of Polyhexanide on Denture Base and Soft Lining Materials

Objective: Polyhexanide (PHMB; polyhexamethylene biguanide) is a well-known antiseptic agent; however, no data exist for its applicationon denture base and lining materials. In the present in vitro study, aim was to compare viable bacterial or fungal cell numbers theiradhesion on different types of materials diverse concentrations PHMB.Methods: Light-activated urethane dimethacrylate (UDMA), heat-polymerized polymethyl methacrylate (PMMA), heat-polymerizedpolydimethylsiloxane, autopolymerizing polyethylmethacrylate discs were prepared each group (n = 10). 1×108 CFU/mL all testedspecies appended separately discs, they immersed into PHMB suspensions (0.5%, 1%, 2%, 5%) 10 minutes.The antimicrobial activity number adherent species surface evaluated.Results: PMMA group, studied except C. albicans, L. acidophilus, S. aureus decreased various (p <0.05), presented significant decrease every concentration UDMA < 0.01) comparison thecontrol. N. sicca, K. pneumoniae, pyogenes, sanguis, pseudotuberculosis, 0.05) reduced heat-polymerizedpolydimethylsiloxane while tested B. subtilis incomparison control 0.01). Among species, difference detected number(p > 0.05).Conclusion: suspension, concentrations, can reduce some yeast cells.